Reporter

Part:BBa_K2605010

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


CMV + mCherry + BGH

Composite part of CMV promoter (Part BBa_K747096) fused to a mCherry reporter gene with BGH terminator (Part BBa_K2605000). This mCherry protein is derived from mushroom coral, Discoma sp. (Shaner et al. 2004).

References

Shaner N.C., Campbell R.E., Steinbach P.A., Giepmans B.N., Palmer A.E., Tsien R.Y. (2004).Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, , 1567-1572

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None